FastQCFastQC Report
Tue 10 Oct 2017
6Hour_CGATGT_L002_R2_003.extract.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6Hour_CGATGT_L002_R2_003.extract.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95868
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAAACTAAACTTATGTGAGTCTTTCTCACTCATGACACCACTTTTC8990.937747736470981No Hit
CCTCCGAACTAAACTTATGTGAGTCTTTCTCACTCATGACACCACTTTTC5970.6227312554762798No Hit
CTCCCCTAATTTCTTTTTTGAACACTCTTCCATTTGGTTGTGTTTCTTCT5550.5789210163975467No Hit
TTCTCATACTTGTTTCTTTCTTCGGCCTCTTGAATTGTGTATGGTTTCTT4700.4902574373096341No Hit
CCCCTAATTTCTTTTTTGAACACTCTTCCATTTGGTTGTGTTTCTTCTTC4490.4683523177702675No Hit
TGGGCCATCTCGCTTCTTTTCAATACTCACAAATACAGTGTTTTCCATAC3560.3713439312387867No Hit
CCCCTGATTTCTTTTTTGAACACTCTTCCATTTGGTTGTGTTTCTTCTTC3520.36717152751700255No Hit
TTCTCGTACTTGTTTCTTTCTTCGGCCTCTTGAATTGTGTATGGTTTCTT3440.3588267200734343No Hit
TGGGCCATCTCGCTTCTTTTCAATACTCACAAATTGAGTGTTTTCCATAC3290.3431802061167439No Hit
GTGCACTTGGTTGTCACTACTTTCTTGGTATTGGTATCTAGCAATGCGAA3120.32544749029916137No Hit
TCCTCCAAACTAAACTTATGTGAGTCTTTCTCACTCATGACACCACTTTT3110.3244043893687153No Hit
TGTTTGAATCGTTGGGGTGTTCCTGGATGGCTTCTACTGAACCAGCATTT3100.3233612884382693No Hit
CCTCCGTCACTTTCTCATACTTGTTTCTTTCTTCGGCCTCTTGAATTGTG2620.27329244377685985No Hit
CGACTACTTATACTATACTACAGTTAAGAGAATAAAAGGTAATGATGACA2430.2534735260983853No Hit
CCTCCATCACTTTCTCATACTTGTTTCTTTCTTCGGCCTCTTGAATTGTG2380.24825802144615514No Hit
TTCTCCTACTTGTTTCTTTCTTCGGCCTCTTGAATTGTGTATGGTTTCTT2230.23261150748946466No Hit
GGGGGTTGATCCAGTTCAGAGCCCAGTGCGCCTTCTTCATGACACAAGGC2210.23052530562857262No Hit
TTCTCTTACTTGTTTCTTTCTTCGGCCTCTTGAATTGTGTATGGTTTCTT2000.20862018608920602No Hit
TGGTTGTCACTACTTTCTTGGTATTGGTATCTAGCAATGCGAACCCATGG1880.19610297492385362No Hit
CGACTGCTTATACTATACTACAGTTAAGAGAATAAAAGGTAATGATGACA1720.17941336003671715No Hit
TGCAGCCACCTCATCTTCTGCTGCTCCTTGATCAGCATCATTAACCTTTT1710.17837025910627113No Hit
CGCCTACTTATACTATACTACAGTTAAGAGAATAAAAGGTAATGATGACA1670.174197855384487No Hit
CCTCTTGAGCATTTACTAAGTCATCAATAAATCCATCATCACCAGCTTCT1660.17315475445404097No Hit
CGTCTAGCTCATCCGAGGATGCCTGGAAACTACTTCTGCTCCACTGGGGC1630.1700254516627029No Hit
ATTCTCTTGGTGGTGATCATGGAGGAACCATTCTCAGAGTGCTCCACAGT1620.16898235073225684No Hit
GTGTGCATGTTTTCACTTCTTCCAGGACAGATTTGCCGTCCTTCTTATTA1590.16585304794091876No Hit
TCCTTCCAGTGTGCATGTTTTCACTTCTTCCAGGACAGATTTGCCGTCCT1560.16272374514958068No Hit
CCTCCTTCACTTTCTCATACTTGTTTCTTTCTTCGGCCTCTTGAATTGTG1520.15855134142779653No Hit
GCTAACTGTGCACTTGGTTGTCACTACTTTCTTGGTATTGGTATCTAGCA1520.15855134142779653No Hit
TTCTTATACTTGTTTCTTTCTTCGGCCTCTTGAATTGTGTATGGTTTCTT1520.15855134142779653No Hit
CCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCT1510.15750824049735052No Hit
CGCTTCTTTTCAATACTCACAAATTGAGTGTTTTCCATACAAATTAAAAA1410.1470772311928902No Hit
CTTGGTGGTGATCATGGAGGAACCATTCTCAGAGTGCTCCACAGTGTGTG1390.14499102933199814No Hit
CGGCTACTTATACTATACTACAGTTAAGAGAATAAAAGGTAATGATGACA1380.14394792840155213No Hit
CGTCTGGCTCATCCGAGGATGCCTGGAAACTACTTCTGCTCCACTGGGGC1370.1429048274711061No Hit
CCAAACTAAACTTATGTGAGTCTTTCTCACTCATGACACCACTTTTCTGG1320.13768932281887594No Hit
CCCCTTATTTCTTTTTTGAACACTCTTCCATTTGGTTGTGTTTCTTCTTC1320.13768932281887594No Hit
CGCTTCTTTTCAATACTCACAAATACAGTGTTTTCCATACAAATGAAAAA1300.13560312095798388No Hit
CTCCCGTAATTTCTTTTTTGAACACTCTTCCATTTGGTTGTGTTTCTTCT1300.13560312095798388No Hit
AGTGTGCATGTTTTCACTTCTTCCAGGACAGATTTGCCGTCCTTCTTATT1290.13456002002753786No Hit
TGGTGGTGATCATGGAGGAACCATTCTCAGAGTGCTCCACAGTGTGTGAC1280.13351691909709182No Hit
AACTGTGCACTTGGTTGTCACTACTTTCTTGGTATTGGTATCTAGCAATG1260.13143071723619978No Hit
TCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTC1250.13038761630575374No Hit
TTCTTGTACTTGTTTCTTTCTTCGGCCTCTTGAATTGTGTATGGTTTCTT1240.12934451537530772No Hit
GCCCGCGATTGTCAGCATGTTGTTCTCAGTAGACAGCAGTATCTTCTGGA1220.12725831351441566No Hit
GTCCAGGTTCTTGATGAAAATGTTTCCCACTCCGGATTTTCGAAGCGAAG1220.12725831351441566No Hit
GGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCA1210.12621521258396962No Hit
CCTCCTCCAAACTAAACTTATGTGAGTCTTTCTCACTCATGACACCACTT1190.12412901072307757No Hit
TGCCGCCACCTCATCTTCTGCTGCTCCTTGATCAGCATCATTAACCTTTT1180.12308590979263154No Hit
CGACCACTTATACTATACTACAGTTAAGAGAATAAAAGGTAATGATGACA1160.12099970793173946No Hit
CGGGGTATTGAATAGGAAGGATCACAAATCGATGAGGGTTTTCCCGGAGA1150.11995660700129344No Hit
CGCCTGCTTATACTATACTACAGTTAAGAGAATAAAAGGTAATGATGACA1140.11891350607084741No Hit
CTGCTGATAAGCTCATTTGAGAATGTCAGACCTGGCATAAGTCCCCTCTT1130.1178704051404014No Hit
CCTCCCTCACTTTCTCATACTTGTTTCTTTCTTCGGCCTCTTGAATTGTG1120.11682730420995535No Hit
GGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCA1100.11474110234906329No Hit
CCTTCCTTCACTTCCACTCCCACCTCTACCGCTGCTGCTGCAGCAGCCTC1050.10952559769683314No Hit
TGGCGTTTCCTTGTGCTCTCATCGTCACCCTCGCCTCGTTTCTCGGATTT1040.10848249676638712No Hit
GTGCGCTTGGTTGTCACTACTTTCTTGGTATTGGTATCTAGCAATGCGAA1040.10848249676638712No Hit
CGCTACTTATACTATACTACAGTTAAGAGAATAAAAGGTAATGATGACAA1010.10535319397504903No Hit
CCTCTCGAGCATTTACTAAGTCATCAATAAATCCATCATCACCAGCTTCT1000.10431009304460301No Hit
TCTCCGTAGGGTTTGGATCACGGGGTGGGTTTGGTCTCTTGAGAGCAAAT990.10326699211415696No Hit
CCACACTAAACTTATGTGAGTCTTTCTCACTCATGACACCACTTTTCTGG990.10326699211415696No Hit
CTCCCTTAATTTCTTTTTTGAACACTCTTCCATTTGGTTGTGTTTCTTCT990.10326699211415696No Hit
CCCCCAATTTCTTTTTTGAACACTCTTCCATTTGGTTGTGTTTCTTCTTC980.10222389118371092No Hit
CCCACCTCTACCGCTGCTGCTGCAGCAGCCTCAGCCTTTCTTTTCTTCTT970.1011807902532649No Hit
TCTCCATAGGGTTTGGATCACGGGGTGGGTTTGGTCTCTTGAGAGCAAAT970.1011807902532649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA201.7312896E-595.048041
TGCGCTT201.7312896E-595.048042
CTCCTAA254.5743764E-795.048042
TCCTAAC201.7312896E-595.048043
CCTAACT201.7312896E-595.048044
CTCTTAC450.095.048043
CGCTACT254.5743764E-795.048041
CTGGCTC254.5743764E-795.048044
GCAGTTG201.7312896E-595.048043
CCCGTAA201.7312896E-595.048043
CCGTAAT201.7312896E-595.048044
CCTCCGA550.095.048041
GCGCTTG201.7312896E-595.048043
GGCTCAT254.5743764E-795.048046
CGAGCAT201.7312896E-595.048046
TCTCTTA450.095.048042
TCCCGTA201.7312896E-595.048042
GTAATTT254.5743764E-795.048046
CGTCTGG201.7312896E-595.048041
CTCCCTG156.586005E-495.0480352
Produced by FastQC (version 0.11.5)